Improper storage is one of the most common causes of peptide degradation. Peptides are sensitive molecules — temperature, moisture, light, and repeated handling all affect compound integrity.
Storage Before Reconstitution (Lyophilised)
| Storage Duration | Temperature |
|---|---|
| Long-term (3+ months) | -20°C (standard freezer) |
| Medium-term (up to 3 months) | 4°C (refrigerator) |
For most research peptides — BPC-157, TB-500, Semaglutide, Tirzepatide — store at -20°C as the default.
Moisture: Keep vials sealed. Never open a cold vial — condensation can enter. Let it reach room temperature first.
Light: Most peptides are light-sensitive. Keep in original packaging until use.
Reconstitution Solvents
| Solvent | Best For |
|---|---|
| Bacteriostatic water | Most research peptides — preferred choice |
| Sterile saline (0.9% NaCl) | Alternative when bacteriostatic water unavailable |
| Acetic acid (0.1%) | Hydrophobic or difficult-to-dissolve peptides |
Bacteriostatic water (sterile water with 0.9% benzyl alcohol) is the standard choice. The benzyl alcohol extends usable life of the reconstituted solution.
Reconstitution Technique
- Allow vial to reach room temperature before opening
- Add solvent slowly — inject down the side of the vial, not directly onto powder
- Do not shake — swirl or roll gently until powder is fully dissolved
- Solution should be clear. Discard if cloudy after full dissolution.
Storage After Reconstitution
| Condition | Stability |
|---|---|
| Refrigerated at 4°C | 2–4 weeks (most peptides) |
| Frozen at -20°C | Not recommended — causes degradation |
| Room temperature | Hours to days only |
Do not freeze reconstituted peptide solutions. Freeze-thaw cycles damage them. Prepare only what you need.
Common Mistakes to Avoid
- Freeze-thaw cycling the reconstituted solution
- Opening a cold vial before it reaches room temperature
- Storing lyophilised peptides at 4°C long-term (use -20°C)
- Vigorous shaking during reconstitution (always swirl gently)
- Using expired bacteriostatic water
Quick Reference Checklist
- ☐ Store lyophilised peptides at -20°C
- ☐ Keep vials sealed until ready to use
- ☐ Let vials reach room temperature before opening
- ☐ Reconstitute with bacteriostatic water
- ☐ Swirl gently — never shake
- ☐ Store reconstituted peptide at 4°C
- ☐ Use reconstituted peptide within 2–4 weeks
- ☐ Do not freeze reconstituted solutions
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